RNA interference in plants

How RNA Interference Combat Viruses in Plants

Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...

Pollen lethality: a phenomenon in Arabidopsis RNA interference plants.

To study gene function, generation of loss-of-function mutants by RNA interference (RNAi) approaches and T-DNA insertional mutagenesis are widely used in plant science and have proven to be very successful in determining gene functions. AGAMOUS-LIKE18 (AGL18) is a MADS-box gene that is expressed during Arabidopsis (Arabidopsis thaliana) pollen development (Alvarez-Buylla et al., 2000; Pina et a...

Targeted Gene Silencing in Plants Using Rna Interference

1 1 Distribution of this document in any form violates the copyrights of the publisher. Cosuppression, post-transcriptional gene silencing (PTGS), and virus-induced gene silencing (VIGS) all describe homology-dependent gene silencing phenomena in plants that involve what is more broadly known as RNA interference (RNAi). The study of these phenomena in plants has provided numerous key discoverie...

an introduction to rna interference (rnai)

the discovery of rna interference (rnai) in recent years has revolutionized biological and medical research. rnai is the sequence-specific gene silencing triggered by double-stranded rna (dsrna). until a couple of years ago only biologists working with caenorhabditis elegans and plants could use rnai technology to gain insight into gene function……